Blog posts

2025

Tumor-Normal Mutation Analysis Pipeline Using GATK Mutect2

3 minute read

Published:

I recently completed a project involving tumor-normal mutation analysis, and I wanted to share my experience with using the GATK Mutect2 pipeline for somatic mutation detection. This was a fantastic learning opportunity that allowed me to explore the intricacies of cancer genomics and the power of next-generation sequencing (NGS). Through this process, I gained a better understanding of how to identify somatic mutations like single nucleotide variants (SNVs) and small insertions/deletions (indels) by comparing tumor and normal samples.